Journal
RSC ADVANCES
Volume 4, Issue 106, Pages 61831-61840Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra11469h
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Funding
- National Hightech R&D Program of China [2012AA030313]
- Changjiang Scholars and Innovative Research Team in University [IRT1135]
- National Natural Science Foundation of China [51203064, 21201083, 51163014]
- Industry-Academia-Research Joint Innovation Fund of Jiangsu Province [BY2012068]
- Science and Technology Support Program of Jiangsu Province [SBE201201094]
- Industry-Academia- Research Prospective Joint Research Program of Jiangsu Province [SBY201220335]
- Innovation Program for Graduate Education in Jiangsu Province [CXZZ13_07]
- Scientific Research Foundation Program for PhD in Jiangnan University [JUDCF13022]
- China Scholarship Council
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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ZnO loaded carbon nanofibers (ZnO/CNFs) were successfully fabricated by a combination of electrospinning, carbonization and a hydrothermal process. A novel biosensor was fabricated based on a composite of ZnO/CNFs, laccase (Lac), and Nafion. The addition of ZnO/CNFs apparently facilitated the direct electron transfer (DET) between the active center of Lac and the surface of the glassy carbon electrode (GCE). A pair of stable and well-defined redox peaks was observed on the Nafion-Lac-ZnO/CNF modified GCE. Meanwhile, square wave voltammetry (SWV) was employed to investigate the biosensor, and the sensor showed highly efficient electrocatalysis toward hydroquinone with a sensitivity of 28.50 mu A mu M-1, a detection limit of 9.50 nM (S/N = 3), a linear range from 5.00 x 10(-7) to 2.06 x 10(-6) M, as well as good selectivity and stability. Furthermore, this novel biosensor was successfully used in detecting hydroquinone in real water samples.
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