4.6 Article

A highly sensitive resonance Rayleigh scattering assay for detection of Hg(II) using immunonanogold as probe

Journal

RSC ADVANCES
Volume 4, Issue 37, Pages 19234-19237

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra02041c

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Funding

  1. National Natural Science Foundation of China [21267004, 21365011, 21367005]
  2. Natural Science Foundation of Guangxi [2013GXNSFFA019003, 2013GXNSFAA019046]

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The monoclonal antibody (mAb) against Hg2+ was produced by the hybridoma technique, and was labeled using nanogold (NG) to obtain an immunonanogold (ING) probe. The immunoreaction takes place to form an ING-Hg2+ immunocomplex that caused the resonance Rayleigh scattering peak at 580 nm to decrease, with a detection limit of 1.1 nmol L-1 Hg2+.

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