Journal
RSC ADVANCES
Volume 2, Issue 3, Pages 968-973Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ra00586c
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Funding
- National Science Council of Taiwan
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Mesoporous silica nanoparticles (MSNs) have received intensive recent interest as multi-functional nanocarriers for biological cells and tissues. Their cell uptake and intracellular distribution depends on the surface charge they carry. We develop a new approach for monitoring their uptake and intracellular localization by measuring the local pH value of the MSNs. We synthesized a ratiometric pH-sensor loaded with mesoporous silica nanoparticles that can map the location of the MSN through the site pH in HeLa cells. Positively charged MSN-TA was found to locate in higher pH regions which are identified as cytosol mostly. On the other hand, negatively charged MSN-PPs were trapped in acidic endosomes. Evidence is given for direct penetration of membranes through a chargemediated membrane-nanoparticle interaction mechanism.
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