Journal
RSC ADVANCES
Volume 2, Issue 10, Pages 4110-4119Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ra20302b
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Funding
- Shanghai-Unilever Research and Development Fund [08520750100]
- National Nature Science Foundation of China [31070871]
- Natural Science Foundation of Shanghai [11ZR1400100]
- Fundamental Research Funds for the Central Universities
- Open Foundation of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials [LK1111, LK0804, LZ0906]
- Program of Introducing Talents of Discipline to Universities [B07024]
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In this work, we reported on the preparation and skin benefits of L-ascorbic acid 2-phosphate (VC-2-p)-loaded silk fibroin (SF) nanofibrous matrices for the first time. The matrices was fabricated using a facile eco-friendly electrospinning processing. With a post treatment of 75 v/v% ethanol vapor, the structure of the matrices transformed from unstable silk I form into water-stable silk II. In vitro release studies confirmed VC-2-p disassociated from SF nanofibrous matrices easily. Both neat and VC-2-p-loaded SF nanofibrous matrices were beneficial to mouse fibroblast L929 cells (L929 cells) adhering, spreading and proliferating against cover slips. Whereas compared with neat SF nanofibrous matrices, VC-2-p-loaded SF nanofibrous matrices significantly promoted the expression of collagen type I alpha 1 (Col1a1), as evidenced by real time PCR. Subsequently, the oxidative injury model further verified both matrices aided L929 cells through antioxidation to survive from tert-butyl hydroperoxide-induced oxidative stress (OS). Importantly, under severe OS, L929 cells on VC-2-p-loaded SF nanofibrous matrices maintained a higher mRNA level of Col1a1 as well as another two important functional genes, glutathione peroxidase 1 and catalase, than neat SF nanofibrous matrices. Our findings clearly indicated that the impressive skin benefits of SF nanofibrous matrices were further reinforced with the incorporation of VC-2-p, which implies the promising application of VC-2-p-loaded SF nanofibrous matrices in personal skin care and skin regeneration, including serving as wound dressings and anti-aging materials.
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