4.6 Article

Experimental and computational insights into the conformations of tunicyclin E, a new cycloheptapeptide from Psammosilene tunicoides

Journal

RSC ADVANCES
Volume 2, Issue 3, Pages 1126-1135

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ra00593f

Keywords

-

Funding

  1. NCET Foundation
  2. National Natural Science Foundation of China [30725045, 20972174, 91029704]
  3. Ministry of Science and Technology, China [2009ZX09308-005, 2009ZX09311-001, 2008ZX09308-005]
  4. Shanghai Leading Academic Discipline Project [B906]
  5. Scientific Foundation of Shanghai, China [09DZ1975700, 09DZ1971500]
  6. State Key Program of Basic Research of China [2009CB918502]

Ask authors/readers for more resources

Tunicyclin E (1), a new cyclic heptapeptide, cyclo(Pro(1)-Ser(2)-Trp(3)-Leu(4)-Val(5)-Gly(6)-Ser(7)), was isolated from the root of Psammosilene tunicoides. The presence of two sets of resonance signals in its NMR spectra (1a:1b, similar to 3 : 1 abundance) indicated that it has two conformations in solution, while only one conformation was found in its crystal state by X-ray diffraction. To explore the molecular basis of the two conformations of 1 in solution and their interconversion mechanism, X-ray diffraction, NMR experiments, and theoretical calculations were performed. The results disclosed that two conformers of 1 in solution were derived from the cis/trans isomers of the Ser(7)-Pro(1) peptide bond (1a, trans; 1b, cis). The fast interconversion of the two conformations in solution is explained by an intramolecular catalysis mechanism and solvent effects. Furthermore, the existence of several unusual pseudo turns characterized for the first time plays a key role for dominant trans conformation in solution.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available