Journal
NUTRIENTS
Volume 6, Issue 2, Pages 564-574Publisher
MDPI AG
DOI: 10.3390/nu6020564
Keywords
oxidative stress; NF-kappa B-iNOS-NO signal pathway; wheat peptides; indomethacin; IEC-6 cells
Categories
Funding
- Postgraduates scientific research and innovation projects in Jiangsu Province [CXZZ12_0124]
- Fundamental Research Funds for the Central Universities
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Recent studies have demonstrated that wheat peptides protected rats against non-steroidal anti-inflammatory drugs-induced small intestinal epithelial cells damage, but the mechanism of action is unclear. In the present study, an indomethacin-induced oxidative stress model was used to investigate the effect of wheat peptides on the nuclear factor-kappa B(NF-kappa B)-inducible nitric oxide synthase-nitric oxide signal pathway in intestinal epithelial cells-6 cells. IEC-6 cells were treated with wheat peptides (0, 125, 500 and 2000 mg/L) for 24 h, followed by 90 mg/L indomethacin for 12 h. Wheat peptides significantly attenuated the indomethacin-induced decrease in superoxide dismutase and glutathione peroxidase activity. Wheat peptides at 2000 mg/L markedly decreased the expression of the NF-kappa B in response to indomethacin-induced oxidative stress. This study demonstrated that the addition of wheat peptides to a culture medium significantly inhibited the indomethacin-induced release of malondialdehyde and nitrogen monoxide, and increased antioxidant enzyme activity in IEC-6 cells, thereby providing a possible explanation for the protective effect proposed for wheat peptides in the prevention of indomethacin-induced oxidative stress in small intestinal epithelial cells.
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