Journal
NUTRIENTS
Volume 1, Issue 2, Pages 263-275Publisher
MDPI
DOI: 10.3390/nu1020263
Keywords
leucine; BCAA; p70(S6K); 4E-BP1; resistance exercise
Categories
Funding
- Dairy Australia [DU1111]
Ask authors/readers for more resources
The effect of resistance exercise with the ingestion of supplementary protein on the activation of the mTOR cascade, in human skeletal muscle has not been fully elucidated. In this study, the impact of a single bout of resistance exercise, immediately followed by a single dose of whey protein isolate (WPI) or placebo supplement, on the activation of mTOR signalling was analyzed. Young untrained men completed a maximal single-legged knee extension exercise bout and were randomized to ingest either WPI supplement (n = 7) or the placebo (n = 7). Muscle biopsies were taken from the vastus lateralis before, and 2, 4 and 24 h post-exercise. WPI or placebo ingestion consumed immediately post-exercise had no impact on the phosphorylation of Akt (Ser(473)). However, WPI significantly enhanced phosphorylation of mTOR (Ser(2448)), 4E-BP1 (Thr(37/46)) and p70(S6K) (Thr(389)) at 2 h post-exercise. This study demonstrates that a single dose of WPI, when consumed in modest quantities, taken immediately after resistance exercise elicits an acute and transient activation of translation initiation within the exercised skeletal muscle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available