Hopane triterpenes as chemotaxonomic markers for the scale insect pathogens Hypocrella s. lat. and Aschersonia

The scale insect pathogens Hypocrella s. lat. and their Aschersonia anamorphs, collected at various locations in Thailand, were surveyed for their productivity of three hopane triterpenes, zeorin (6 alpha,22-dihydroxyhopane), dustanin (15 alpha,22-dihydroxyhopane), and 3 beta-acetoxy-15 alpha,22-dihydroxyhopane, when cultured in a liquid medium (potato-dextrose broth) under static conditions. Among 53 strains of Aschersonia species, 48 strains (91 %) produced at least one of these compounds. Hypocrella and Moelleriella species (43 strains) also frequently produced these triterpenoids; only two strains lacked all of these triterpenes. The results demonstrate that hopane triterpenes may be suitable for use as chemotaxonomic markers for Hypocrella and Moelleriella species and their Aschersonia anamorphs. (C) 2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.