Journal
MATERIALS
Volume 7, Issue 3, Pages 2104-2119Publisher
MDPI
DOI: 10.3390/ma7032104
Keywords
silk fibroin; indirect additive manufacturing technology; scaffold; chondrocytes; cartilage tissue engineering selective laser sintering
Categories
Funding
- National Science Council [NSC98-2314-B-182A-026-MY2, NSC102-2320-B-182-004-MY3]
- Chang Gung Memorial Hospital [CRRPD5C0201]
Ask authors/readers for more resources
Advanced tissue engineering (TE) technology based on additive manufacturing (AM) can fabricate scaffolds with a three-dimensional (3D) environment suitable for cartilage regeneration. Specifically, AM technology may allow the incorporation of complex architectural features. The present study involves the fabrication of 3D TE scaffolds by an indirect AM approach using silk fibroin (SF). From scanning electron microscopic observations, the presence of micro-pores and interconnected channels within the scaffold could be verified, resulting in a TE scaffold with both micro-and macro-structural features. The intrinsic properties, such as the chemical structure and thermal characteristics of SF, were preserved after the indirect AM manufacturing process. In vitro cell culture within the SF scaffold using porcine articular chondrocytes showed a steady increase in cell numbers up to Day 14. The specific production (per cell basis) of the cartilage-specific extracellular matrix component (collagen Type II) was enhanced with culture time up to 12 weeks, indicating the re-differentiation of chondrocytes within the scaffold. Subcutaneous implantation of the scaffold-chondrocyte constructs in nude mice also confirmed the formation of ectopic cartilage by histological examination and immunostaining.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available