Journal
JOURNAL OF DIABETES INVESTIGATION
Volume 5, Issue 1, Pages 31-37Publisher
WILEY
DOI: 10.1111/jdi.12129
Keywords
Gastric inhibitory polypeptide; Incretins; Peripheral nervous system
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [23591303]
- Grants-in-Aid for Scientific Research [23591303, 24659451, 25293210] Funding Source: KAKEN
Ask authors/readers for more resources
Aims/IntroductionGastric inhibitory polypeptide (GIP) is an incretin secreted from the gastrointestinal tract after an ingestion of nutrients, and stimulates an insulin secretion from the pancreatic islets. Additionally, GIP has important roles in extrapancreatic tissues: fat accumulation in adipose tissue, neuroprotective effects in the central nervous system and an inhibition of bone resorption. In the current study, we investigated the effects of GIP signaling on the peripheral nervous system (PNS). Materials and MethodsFirst, the presence of the GIP receptor (GIPR) in mouse dorsal root ganglion (DRG) was evaluated utilizing immunohistochemical analysis, western blotting and reverse transcription polymerase chain reaction. DRG neurons of male wild-type mice (WT) were cultured with or without GIP, and their neurite lengths were quantified. Functions of the PNS were evaluated in GIPR-deficient mice (gipr-/-) and WT by using current perception thresholds (CPTs), Thermal Plantar Test (TPT), and motor (MNCV) and sensory nerve conduction velocity (SNCV, respectively). Sciatic nerve blood flow (SNBF) and plantar skin blood flow (PSBF) were also evaluated. ResultsWe confirmed the expression of GIPR in DRG neurons. The neurite outgrowths of DRG neurons were promoted by the GIP administrations. The gipr-/- showed impaired perception functions in the examination of CPTs and TPT. Both MNCV and SNCV were delayed in gipr-/- compared with these in WT. There was no difference in SNBF and PSBF between WT and gipr-/-. ConclusionsOur findings show that the GIP signal could exert direct physiological roles in the PNS, which might be directly exerted on the PNS.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available