Journal
PATHOGENS AND DISEASE
Volume 74, Issue 2, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femspd/ftv123
Keywords
STEC; O157:H7; Congo red; biofilm; rcsB; mlrA
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Funding
- United States Department of Agriculture, Agricultural Research Service, National Program 108 Food Safety [CRIS] [8072-42000-067]
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High variability in the expression of csgD-dependent, biofilm-forming and adhesive properties is common among Shiga toxin-producing Escherichia coli. Although many strains of serotype O157:H7 form little biofilm, conversion to stronger biofilm phenotypes has been observed. In this study, we screened different strains of serotype O157:H7 for the emergence of strong Congo-red (CR) affinity/biofilm-forming properties and investigated the underlying genetic mechanisms. Two major mechanisms which conferred stronger biofilm phenotypes were identified:mutations (insertion, deletion, single nucleotide change) in rcsB region and stx-prophage excision from the mlrA site. Restoration of the native mlrA gene (due to prophage excision) resulted in strong biofilm properties to all variants. Whereas RcsB mutants showed weaker CR affinity and biofilm properties, it provided more possibilities for phenotypic presentations through heterogenic sequence mutations.
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