4.2 Article

Development and characterization of 22 microsatellite loci for the ringed salamander (Ambystoma annulatum) using paired-end Illumina shotgun sequencing

Journal

CONSERVATION GENETICS RESOURCES
Volume 5, Issue 4, Pages 993-995

Publisher

SPRINGER
DOI: 10.1007/s12686-013-9951-3

Keywords

Ambystoma; Population genetics; Landscape genetics; Multiplex PCR; PAL_Finder

Funding

  1. DoD Strategic Environmental Research and Development Program [RC2155]

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We isolated and screened 150 microsatellite loci from the ringed salamander, Ambystoma annulatum, an ambystomatid salamander endemic to the Interior Highlands. Twenty-two polymorphic loci were identified and pooled into two multiplex reactions. These loci will be valuable tools for assessing population genetic structure and connectivity across the landscape, and informing management of this species.

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