Isolation and characterization of twenty tetranucleotide microsatellite DNA makers in a schizothoracin fish, Schizothorax prenanti (Tchang)

Prenant's schizothoracin, Schizothorax prenanti (Tchang) is an important polyploidy fish in upper reaches of the Yangtze River. Given the extreme anthropogenic environmental alteration in the region, S. prenanti represents an excellent model organism to study historical and contemporary environmental changes. We developed twenty tetranucleotide microsatellite DNA markers from a tetranucleotide microsatellite enriched library. These highly polymorphic markers (2-23 alleles) will facilitate the population genetic analyses of this species.