Characterization of tetranucleotide microsatellite loci for Olympic mudminnow (Novumbra hubbsi)

We describe the isolation and amplification of 21 potential tetranucleotide microsatellites for the Olympic mudminnow (Novumbra hubbsi). After initial testing, eight microsatellites were removed from future analysis for non-resolvable electropherograms. The remaining 13 loci were screened against a total of 772 individuals from 12 populations. The thirteen polymorphic loci amplified between four and forty-eight alleles per locus with expected heterozygosities ranging from 0.41 to 0.95. These markers should aid in conservation and the description of population structure of this state sensitive species.