Isolation and characterisation of microsatellite markers from the South Island robin (Petroica australis)

We identified 20 novel polymorphic microsatellite markers for use in South Island robins (Petroica australis). Markers were isolated from clones enriched for microsatellite repeats, and using 454 pyro-sequencing. South Island robins, like many New Zealand endemic species, have relatively low microsatellite diversity. Alleles per locus ranged from 2 to 7 (mean 3.00, SE 0.299). Mean expected heterozygosity was 0.436 (SE 0.047). Markers were amplified using cost-effective universal fluorescent labeling in multiplex PCR and will be useful in conservation genetics research involving this locally threatened species.