Journal
CONSERVATION GENETICS RESOURCES
Volume 5, Issue 2, Pages 327-330Publisher
SPRINGER
DOI: 10.1007/s12686-012-9795-2
Keywords
Poaching; Corallium rubrum; Dried tissues; Degraded DNA; DNA extraction; Microsatellites; Genotyping
Categories
Funding
- Provence-Al-pes-Cote d'Azur'' region
- IFB Foundation (Institut Francais de la Biodiversite)
- environmental department of TOTAL-FINA-ELF group
- 'Action Concertee Initiative' (ACI) program 'Jeunes chercheurs 2003' French Research Ministry [JC5360]
- Agence Nationale pour la Recherche (ANR)
- Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BPD/74400/2010]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/74400/2010] Funding Source: FCT
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Corallium rubrum is an overharvested precious coral submitted to strong environmental pressures. The development of new management tools such as assignment tests is crucial to assess the origin of traded colonies and to reinforce existing regulations in order to ultimately reduce poaching. As a starting point, we test the feasibility of DNA extractions and PCR amplifications of ten microsatellites using dried tissues such as those that can be obtained from traded or seized colonies. We genotype the same ten colonies conserved in alcohol and after five drying periods (from 7 days to 8 months) as well as ten dried colonies coming from a seizure conducted in 2009. Estimating the rate of negative PCRs through time, PCR repeatability and frequencies of null alleles, we demonstrate that dried colonies can be successfully genotyped. This study opens new avenues for the conservation of C. rubrum and other precious corals.
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