Journal
CELL MOTILITY AND THE CYTOSKELETON
Volume 66, Issue 8, Pages 556-566Publisher
WILEY-LISS
DOI: 10.1002/cm.20365
Keywords
myofibrillogenesis; myofibril; alpha-actinin; myosin; A-bands; premyofibril; nascent myofibril; mature myofibril; fluorescence recovery after photobleaching; FRAP
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Funding
- NCRR NIH HHS [S10 RR017879-01] Funding Source: Medline
- NHLBI NIH HHS [R01 HL080426, R01 HL080426-05, R01 HL048954-13, R01 HL048954] Funding Source: Medline
- NIAMS NIH HHS [R01 AR046481-06, R01 AR046481] Funding Source: Medline
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The premyofibril model of myofibrillogenesis, based on observations in cultured avian muscle cells, proposes that mature myofibrils are preceded by two intermediary structures: premyofibrils and nascent myofibrils. To determine if this model applies to zebrafish skeletal muscle development, we stained developing embryos with antibodies to sarcomeric alpha-actinin and myosin II. In the youngest muscle cells, sarcomeric alpha-actinin and non-muscle myosin II were each localized in linear arrays of small bands that resembled the premyofibrils in avian myocytes. The distribution of muscle-specific myosin II began as scattered short filaments followed in time by overlapping bundles of filaments and organized A-bands in the older somites. Alpha-actinin organization changed from small z-bodies to beaded Z-bands and ordered Z-bands in myofibrils that extended the length of the elongating somites. In older somites with mature myofibrils, premyofibrils were also present at the ends of the mature myofibrils, suggesting that as the cells and somites grew longer, premyofibrils were involved in the elongation of existing mature myofibrils. Fluorescence Recovery After Photobleaching showed that the exchange of proteins (actin, alpha-actinin, FATZ, myotilin and telethonin) between sarcoplasm and the Z-bands of mature myofibrils in zebrafish resembled that seen for the same proteins in cultured avian myotubes, suggesting that myofibril assembly and maintenance in zebrafish share common properties with avian muscle. Cell Motil. Cytoskeleton 66: 556-566, 2009. (C) 2009 Wiley-Liss, Inc.
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