Journal
CYTOSKELETON
Volume 67, Issue 1, Pages 13-22Publisher
WILEY
DOI: 10.1002/cm.20412
Keywords
cytokinesis; cytokinetic apparatus; isolation and partial purification; actomyosin ring; Saccharomyces cerevisiae
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Funding
- Swiss National Science Foundation (SNF)
- NIH [GM42759]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM042759, R01GM042759] Funding Source: NIH RePORTER
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Cytokinesis is the process by which a cell physically divides in two at the conclusion of a cell cycle. In animal and fungal cells, this process is mediated by a conserved set of proteins including actin, type II myosin, IQGAP proteins, F-BAR proteins, and the septins. To facilitate biochemical and ultrastructural analysis of cytokinesis, we have isolated and partially purified the Saccharomyces cerevisiae cytokinetic apparatus. The isolated apparatus contains all components of the actomyosin ring for which we tested actin, myosin heavy and light chain, and IQGAP as well as septins and the cytokinetic F-BAR protein, Hoflp. We also present evidence indicating that the actomyosin rings associated with isolated cytokinetic apparati may be contractile in vitro, and show preliminary electron microscopic imaging of the cytokinetic apparatus. This first successful isolation of the cytokinetic apparatus from a genetically tractable organism promises to make possible a deeper understanding of cytokinesis. (C) 2009 Wiley-Liss, Inc.
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