Journal
CELL ADHESION & MIGRATION
Volume 5, Issue 4, Pages 344-350Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cam.5.4.16717
Keywords
lumen; N-WASP; tuba; E-cadherin; pre-apical patch
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Funding
- National Health and Medical Council (NHMRC) of Australia
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The process of epithelial lumenogenesis requires coordination of a network of signaling machinery communicated to each cell through subsequent cell divisions. Formation of a single hollow lumen has previously been shown to require Tuba, a Cdc42 GEF, for Cdc42 activation and correct spindle orientation. Using a Caco-2 model of lumenogenesis, we show that knockdown (KD) of the actin regulator N-WASP, causes a multilumen phenotype similar to Tuba KD. Defects in lumenogenesis in Tuba KD and N-WA SP KD cells are observed at the two cell stage with inappropriate marking of the pre-apical patch (PAP)-the precursor to lumen formation. Strikingly, both Tuba and N-WA SP depend on each other for localization to the PAP. We conclude that N-WA SP functions cooperatively with Tuba to facilitate lumenogenesis and this requires the polyproline region of N-WASP.
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