Journal
CELL ADHESION & MIGRATION
Volume 4, Issue 3, Pages 431-438Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cam.4.3.12138
Keywords
neural crest; Wnt; cadherin-7; cadherin-11
Categories
Funding
- NICHD
- NIH Center of Biomedical Research Excellence (COBRE) [P20 RR015567]
- USD Research Catalyst Grant
- NIH of the National Center for Research Resources [2 P20 RR016479]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0923419] Funding Source: National Science Foundation
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In neural crest cell development, the expression of the cell adhesion proteins cadherin-7 and cadherin-11 commences after delamination of the neural crest cells from the neuroepithelium. The canonical Wnt signaling pathway is known to drive this delamination step and is a candidate for inducing expression of these cadherins at this time. This project was initiated to investigate the role of canonical Wnt signaling in the expression of cadherin-7 and cadherin-11 by treating neural crest cells with Wnt3a ligand. Expression of cadherin-11 was first confirmed in the neural crest cells for the chicken embryo. The changes in the expression level of cadherin-7 and -11 following the treatment with Wnt3a were studied using real-time RT-PCR and immunostaining. Statistically significant upregulation in the mRNA expression of cadherin-7 and cadherin-11 and in the amount of cadherin-7 and cadherin-11 protein found in cell-cell interfaces between neural crest cells was observed in response to Wnt, demonstrating that cadherin-7 and cadherin-11 expressed by the migrating neural crest cells can be regulated by the canonical Wnt pathway.
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