Journal
CELL ADHESION & MIGRATION
Volume 4, Issue 4, Pages 491-501Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/cam.4.4.12661
Keywords
E-cadherin; adherens junctions; plasma membrane; protein dynamics; photoactivation; photobleaching
Categories
Funding
- Cancer Research UK [C157/A9148]
- Fundacion Espanola para la Ciencia y la Tecnologia
Ask authors/readers for more resources
The dynamic control of E-cadherin is critical for establishing and maintaining cell-cell junctions in epithelial cells. The concentration of E-cadherin molecules at adherens junctions (AJs) is regulated by lateral movement of E-cadherin within the plasma membrane and endocytosis. Here we set out to study the interplay between these processes and their contribution to E-cadherin dynamics. Using photoactivation (PA) and fluorescence recovery after photobleaching (FRAP), we were able to monitor the fate of E-cadherin molecules within the plasma membrane. Our results suggest that the motility of E-cadherin within and away from the cell surface are not exclusive or independent mechanisms and there is a fine balance between the two which, when perturbed, can have dramatic effects on the regulation of AJs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available