Journal
CATALYSIS SCIENCE & TECHNOLOGY
Volume 3, Issue 10, Pages 2761-2768Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cy00268c
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [41003040, 41201487]
- Natural Science Foundation of Tianjin [10JCYBJC06000]
- Program of Introducing Talents of Discipline to Universities [B06006]
Ask authors/readers for more resources
A composite material has been successfully prepared for the first time using an amino-containing metal-organic framework (MOF) as a new type of host matrix material to anchor hemin and simulate the peptidic microenvironment of the native peroxidase. The material was characterized by XRD, SEM, EDS, FT-IR techniques and an N-2 adsorption method. It exhibited peroxidase-like activity through catalytic oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, producing a blue-colored solution. Under optimal conditions, the absorbance at 652 nm is linearly correlated with the concentration of H2O2 from 5.0 x 10(-6) mol L-1 to 2.0 x 10(-4) mol L-1 (R-2 = 0.994). More importantly, a sensitive and selective method for glucose detection was developed using the as-prepared mimetic peroxidase as a catalyst with the help of glucose oxidase (GOx). The analytical platform for glucose detection was observed to have a linear range from 1.0 x 10(-5) mol L-1 to 3.0 x 10(-4) mol L-1 (R-2 = 0.993). This work informs researchers of the advantages of MOFs for preparing biomimetic catalysts and extends the functionality of MOFs to biosensor applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available