Journal
BIOMEDICAL OPTICS EXPRESS
Volume 5, Issue 8, Pages 2517-2526Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.002517
Keywords
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Funding
- NSF [MRI 1039562]
- Chamber's Fellowship
- Pratt-Gardner Fellowship
- Rothschild Fellowship
- Weizmann Institute of Science - national postdoctoral award program for advancing women in science
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1039562] Funding Source: National Science Foundation
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We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanoparticles (NPs) on multiple substrates, including a monolayer of NPs on a silanized coverslip, and NPs bound to live cells. Furthermore, the set up allowed for co-registered quantitative phase imaging (QPI) to be acquired in an off-axis holographic set-up. An SNR of 103 was obtained for NP-tagging of epidermal growth factor receptor (EGFR) in live cells with a 3 second acquisition, while an SNR of 47 was seen for 20 ms acquisition. An analysis of improvements in SNR due to averaging multiple frames is presented, which suggest that residual photothermal signal can be a limiting factor. The combination of techniques allows for high resolution imaging of cell structure via QPI with the ability to identify receptor expression via POLI. (C) 2014 Optical Society of America
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