Journal
BIOMEDICAL OPTICS EXPRESS
Volume 5, Issue 11, Pages 4076-4088Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.004076
Keywords
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Funding
- National Basic Research Program of China (973 Program) [2013CB834704, 2011CB503700]
- National Natural Science Foundation of China [61275190, 91233208]
- Program of Zhejiang Leading Team of Science and Technology Innovation [2010R50007]
- Fundamental Research Funds for the Central Universities
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Near-infrared (NIR) imaging technology has been widely used for biomedical research and applications, since it can achieve deep penetration in biological tissues due to less absorption and scattering of NIR light. In our research, polymer nanoparticles with NIR fluorophores doped were synthesized. The morphology, absorption/emission features and chemical stability of the fluorescent nanoparticles were characterized, separately. NIR fluorescent nanoparticles were then utilized as bright optical probes for macro in vivo imaging of mice, including sentinel lymph node (SLN) mapping, as well as distribution and excretion monitoring of nanoparticles in animal body. Furthermore, we applied the NIR fluorescent nanoparticles in in vivo microscopic bioimaging via a confocal microscope. Under the 635 nm-CW excitation, the blood vessel architecture in the ear and the brain of mice, which were administered with nanoparticles, was visualized very clearly. The imaging depth of our one-photon microscopy, which was assisted with NIR fluorescent nanoprobes, can reach as deep as 500 mu m. Our experiments show that NIR fluorescent nanoparticles have great potentials in various deep-tissue imaging applications. (C) 2014 Optical Society of America
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