Journal
BIOMEDICAL OPTICS EXPRESS
Volume 5, Issue 10, Pages 3292-3304Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.003292
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Funding
- National Eye Institute (NEI) [R21-EY023451]
- National Cancer Institute (NCI) [R01-CA167421]
- NIH Training Grant (NIBIB) [T32EB001040]
- Pratt Gardner Graduate Fellowship
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We present a fast approach for size determination of spherical scatterers using the continuous wavelet transform of the angular light scattering profile to address the computational limitations of previously developed sizing techniques. The potential accuracy, speed, and robustness of the algorithm were determined in simulated models of scattering by polystyrene beads and cells. The algorithm was tested experimentally on angular light scattering data from polystyrene bead phantoms and MCF-7 breast cancer cells using a 2D a/LCI system. Theoretical sizing of simulated profiles of beads and cells produced strong fits between calculated and actual size (r(2) = 0.9969 and r(2) = 0.9979 respectively), and experimental size determinations were accurate to within one micron. (C)2014 Optical Society of America
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