Journal
BIOMEDICAL OPTICS EXPRESS
Volume 5, Issue 1, Pages 233-243Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.000233
Keywords
-
Funding
- Arthritis Research UK [20299]
- NIHR Biomedical Research Unit into Musculoskeletal Disease
- Canada Foundation for Innovation
- Canadian Institute for Photonic Innovations
- Natural Sciences and Engineering Research Council of Canada
- Mprime NCE Strategic Postdoctoral Research Program
- Ministere du Developpement economique, Innovation et Exportation
- Fonds de recherche du Quebec-Nature et technologies
- Cancer Research UK
- Versus Arthritis [18887] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0611-10216] Funding Source: researchfish
- Orthopaedic Research UK [458] Funding Source: researchfish
- Versus Arthritis [20299] Funding Source: researchfish
Ask authors/readers for more resources
The collagen meshwork plays a central role in the functioning of a range of tissues including cartilage, tendon, arteries, skin, bone and ligament. Because of its importance in function, it is of considerable interest for studying development, disease and regeneration processes. Here, we have used second harmonic generation (SHG) to image human tissues on the hundreds of micron scale, and developed a numerical model to quantitatively interpret the images in terms of the underlying collagen structure on the tens to hundreds of nanometer scale. Focusing on osteoarthritic changes in cartilage, we have demonstrated that this combination of polarized SHG imaging and numerical modeling can estimate fibril diameter, filling fraction, orientation and bundling. This extends SHG microscopy from a qualitative to quantitative imaging technique, providing a label-free and non-destructive platform for characterizing the extracellular matrix that can expand our understanding of the structural mechanisms in disease. (C) 2013 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available