Journal
BIOMEDICAL OPTICS EXPRESS
Volume 4, Issue 11, Pages 2376-2382Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.4.002376
Keywords
-
Funding
- NSF CAREER Award [CBET-1151154]
- NASA Early Career Faculty Grant [NNX12AQ44G]
- Gulf of Mexico Research Initiative grant [GoMRI-030]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1151154] Funding Source: National Science Foundation
Ask authors/readers for more resources
We present high-speed hyperspectral Raman imaging with integrated active-illumination for label-free compositional microanalysis. We show that high-quality Raman spectra can be acquired from as many as similar to 1,000 spots/sec semi-randomly distributed among a similar to 100x100 mu m(2) area without mechanical scanning. We demonstrate rapid data acquisition from three types of samples: 1) uniform, strong Raman scatterers, e.g., silicon substrates; 2) non-uniform, medium-strength Raman scatterers, e.g., polymer microparticles; and, 3) non-uniform, relatively weak Raman scatterers, e.g., bacterial spores. We compare the system performance to that of point-scan with an electron-multiplied CCD camera, as implemented in some commercial systems. The results suggest that our system not only provides significant imaging speed advantage for various types of samples, but also permits substantially longer integration time per spot, leading to superior signal-to-noise ratio data. Our system enables the rapid collection of high quality Raman spectra for reliable and robust compositional microanalysis that are potentially transformative in applications such as semiconductor material and device, polymer blend and biomedicine. (C) 2013 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available