Journal
BIOMEDICAL OPTICS EXPRESS
Volume 4, Issue 12, Pages 2869-2879Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.4.002869
Keywords
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Funding
- European Research Council [SINSLIM 258221]
- Laboratoire Europeen Associe NaBi
- Human Frontier Science Program [RGP0013/2010]
- 'Fondation pour la Recherche Medicale' (FRM Equipe)
- 'Agence Nationale de la Recherche' [ANR-12-BSV5-0011-01, ANR-10-INSB-04-01]
- Ecole des Neurosciences de Paris (ENP)
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The use of wavefront shaping to generate extended optical excitation patterns which are confined to a predetermined volume has become commonplace on various microscopy applications. For multiphoton excitation, three-dimensional confinement can be achieved by combining the technique of temporal focusing of ultra-short pulses with different approaches for lateral light shaping, including computer generated holography or generalized phase contrast. Here we present a theoretical and experimental study on the effect of scattering on the propagation of holographic beams with and without temporal focusing. Results from fixed and acute cortical slices show that temporally focused spatial patterns are extremely robust against the effects of scattering and this permits their three-dimensionally confined excitation for depths more than 500 mu m. Finally we prove the efficiency of using temporally focused holographic beams in two-photon stimulation of neurons expressing the red-shifted optogenetic channel C1V1. (C) 2013 Optical Society of America
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