Journal
BIOMEDICAL OPTICS EXPRESS
Volume 4, Issue 6, Pages 909-916Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.4.000909
Keywords
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Funding
- National Science Council [NSC-98-2112-M-002-003-MY3, NSC-101-2923-M-002-001-MY3]
- Academy of Finland [134973]
- Graduate School of Modern Optics and Photonics in Finland
- Emil Aaltonen Foundation
- Academy of Finland (AKA) [134973] Funding Source: Academy of Finland (AKA)
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We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules. (C) 2013 Optical Society of America
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