4.6 Article

Re-scan confocal microscopy: scanning twice for better resolution

Journal

BIOMEDICAL OPTICS EXPRESS
Volume 4, Issue 11, Pages 2644-2656

Publisher

OPTICAL SOC AMER
DOI: 10.1364/BOE.4.002644

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Funding

  1. Dutch Technology Foundation STW, of the Netherlands Organisation for Scientific Research (NWO)
  2. Ministry of Economic Affairs
  3. Dutch Technology Foundation STW project [11350, 12151]

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We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high sensitivity is required. (C) 2013 Optical Society of America

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