Journal
BIOMEDICAL OPTICS EXPRESS
Volume 3, Issue 7, Pages 1492-1505Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.3.001492
Keywords
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Funding
- Generalitat de Catalunya [2009-SGR-159]
- Spanish government [TEC2009-09698, SAF2008-00211]
- EU project STELUM [217997]
- Swiss National Science Foundation (SNSF) Sinergia project grant [CRSII3_125447]
- FP7-NMP VIBRANT project grant [CP-IP 228933-2]
- NoE P4L
- Laserlab optobio
- CONACYT Mexico
- Fundacio Cellex Barcelona
- ICREA Funding Source: Custom
- Swiss National Science Foundation (SNF) [CRSII3_125447] Funding Source: Swiss National Science Foundation (SNF)
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We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivo Caenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view. (C) 2012 Optical Society of America
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