Journal
BIOMEDICAL OPTICS EXPRESS
Volume 3, Issue 11, Pages 2860-2865Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.3.002860
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Funding
- National Science Council Taiwan [NSC 100-2628-E-002-006]
- National Health Research Institutes [NHRI-EX101-9936EI]
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With a video-rate third harmonic generation (THG) microscopy system, we imaged the micro-circulation beneath the human skin without labeling. Not only the speed of circulation but also the morpho-hydrodynamics of blood cells can be analyzed. Lacking of nuclei, red blood cells (RBCs) shows typical parachute-like and hollow-core morphology under THG microscopy. Quite different from RBCs, every now and then, round and granule rich blood cells with strong THG contrast appear in circulation. The corresponding volume densities in blood, evaluated from their frequencies of appearance and the velocity of circulation, fall within the physiological range of human white blood cell counts. (C) 2012 Optical Society of America
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