Journal
BIOMEDICAL OPTICS EXPRESS
Volume 3, Issue 11, Pages 2752-2760Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.3.002752
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Funding
- Duke University
- NIH [1RC1CA145105]
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Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators. (C) 2012 Optical Society of America
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