Journal
BIOMEDICAL OPTICS EXPRESS
Volume 3, Issue 11, Pages 2825-2841Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.3.002825
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Funding
- National Science Foundation [CBET-0756005]
- Korea Military Academy Hwarangdae Research Institute
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Coherence-gated dynamic light scattering captures cellular dynamics through ultra-low-frequency (0.005-5 Hz) speckle fluctuations and Doppler shifts that encode a broad range of cellular and subcellular motions. The dynamic physiological response of tissues to applied drugs is the basis for a new type of phenotypic profiling for drug screening on multicellular tumor spheroids. Volumetrically resolved tissue-response fluctuation spectrograms act as fingerprints that are segmented through feature masks into high-dimensional feature vectors. Drug-response clustering is achieved through multidimensional scaling with simulated annealing to construct phenotypic drug profiles that cluster drugs with similar responses. Hypoxic vs. normoxic tissue responses present two distinct phenotypes with differentiated responses to environmental perturbations and to pharmacological doses. (C) 2012 Optical Society of America
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