Journal
BIOMEDICAL OPTICS EXPRESS
Volume 2, Issue 3, Pages 408-420Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.2.000408
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Funding
- Swiss National Science Foundation SNSF [CRSII3 125463, K-23K1-116242/1, 315200-116729]
- Swiss National Science Foundation (SNF) [CRSII3_125463] Funding Source: Swiss National Science Foundation (SNF)
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A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time using high-precision localization algorithms (e. g., PALM and STORM) or by analyzing the statistics of the temporal fluctuations (SOFI). In a face-to-face comparison of the two post-processing algorithms, we show that localization-based super-resolution can deliver higher resolution enhancements but imposes significant constraints on the blinking behavior of the probes, which limits its applicability for live-cell imaging. SOFI, on the other hand, works more consistently over different photo-switching kinetics and also delivers information about the specific blinking statistics. Its suitability for low SNR acquisition reveals SOFI's potential as a high-speed super-resolution imaging technique. (C) 2011 Optical Society of America
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