Journal
BIOMEDICAL OPTICS EXPRESS
Volume 2, Issue 12, Pages 3387-3392Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.2.003387
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Funding
- United States Army and Defense Advanced Research Projects Agency
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High-speed high-contrast imaging modalities that enable image acquisition of transparent media without the need for chemical staining are essential tools for a broad range of applications; from semiconductor process monitoring to blood screening. Here we introduce a method for contrast-enhanced imaging of unstained transparent objects that is capable of high-throughput imaging. This method combines the Nomarski phase contrast capability with the ultrahigh frame rate and shutter speed of serial time-encoded amplified microscopy. As a proof of concept, we show imaging of a transparent test structure and white blood cells in flow at a shutter speed of 33 ps and a frame rate of 36.1 MHz using a single-pixel photo-detector. This method is expected to be a valuable tool for high-throughput screening of unstained cells. (C) 2011 Optical Society of America
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