Journal
BIOMEDICAL OPTICS EXPRESS
Volume 2, Issue 11, Pages 3135-3149Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.2.003135
Keywords
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Funding
- Generalitat de Catalunya [2009-SGR-159]
- Spanish government [TEC2009-09698, SAF2008-00211]
- EU [FP7-PEOPLE-2007-3-1-IAPP, 217997]
- NoE P4L and Laserlab optobio
- Fundacio Cellex Barcelona
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We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded using a Shack-Hartmann sensor. Compensation of the recorded sample aberrations is performed by the deformable mirror in a single-step. This technique is applied to fixed and in vivo biological samples, showing, in some cases, more than one order of magnitude improvement in the total collected signal intensity. (C) 2011 Optical Society of America
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