Journal
BIOMEDICAL OPTICS EXPRESS
Volume 2, Issue 9, Pages 2590-2598Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.2.002590
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Funding
- National Science Foundation [IOB-0545679]
- Human Frontier Science Program [RGP0021/2007C]
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We use a spatial light modulator (SLM) to diffract a single UV laser pulse to ablate multiple points on a Drosophila embryo. This system dynamically generates a phase hologram for ablating a user-defined pattern fast enough to be used with living, and thus moving, tissue. We demonstrate the ability of this single-pulse multi-point system to perform two experiments that are very difficult for conventional microsurgery-isolating single cells in vivo and measuring fast retractions from large incisions. (C) 2011 Optical Society of America
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