Journal
BIOMEDICAL OPTICS EXPRESS
Volume 2, Issue 2, Pages 365-373Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.2.000365
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An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 x 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed. (C) 2011 Optical Society of America
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