Journal
BIOMEDICAL OPTICS EXPRESS
Volume 2, Issue 4, Pages 981-986Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.2.000981
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Funding
- NIH [P30 DK034993, P50 CA93990, R01 CA142750]
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We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bundle with outer diameter of 680 mu m to collect en face images at 10 Hz with 4 mu m lateral resolution. We applied the FITC-labeled peptide QPIHPNNM topically to colonic mucosa in genetically engineered mice that spontaneously develop adenomas. More than two-fold greater fluorescence intensity was measured from adenomas compared to adjacent normal-appearing mucosa. Images of adenomas showed irregular morphology characteristic of dysplasia. (C) 2011 Optical Society of America
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