Journal
BIOMEDICAL OPTICS EXPRESS
Volume 1, Issue 2, Pages 453-462Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.1.000453
Keywords
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Funding
- National Institutes of Health [1 R15 EB008858-01]
- U.S. Civilian Research and Development Foundation [UKB2-2923-KV-07]
- Ministry of Education and Science of Ukraine [M/49-2008]
- National Science Foundation [CHE-0832622, CHE-0840431]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0840431] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [934212] Funding Source: National Science Foundation
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Narrow dispersity organically modified silica nanoparticles (SiNPs), diameter similar to 30 nm, entrapping a hydrophobic two-photon absorbing fluorenyl dye, were synthesized by hydrolysis of triethoxyvinylsilane and (3-aminopropyl) triethoxysilane in the nonpolar core of Aerosol-OT micelles. The surface of the SiNPs were functionalized with folic acid, to specifically deliver the probe to folate receptor (FR) over-expressing Hela cells, making these folate two-photon dye-doped SiNPs potential candidates as probes for two-photon fluorescence microscopy (2PFM) bioimaging. In vitro studies using FR over-expressing Hela cells and low FR expressing MG63 cells demonstrated specific cellular uptake of the functionalized nanoparticles. One-photon fluorescence microscopy (1PFM) imaging, 2PFM imaging, and two-photon fluorescence lifetime microscopy (2P-FLIM) imaging of Hela cells incubated with folate-modified two-photon dye-doped SiNPs were demonstrated. (C) 2010 Optical Society of America
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