Journal
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
Volume 5, Issue -, Pages 346-354Publisher
BEILSTEIN-INSTITUT
DOI: 10.3762/bjnano.5.39
Keywords
biosensor; carbon nanofibers; catechol; electrospinning; laccase
Funding
- National High-tech R&D Program of China [2012AA030313]
- Changjiang Scholars and Innovative Research Team in University [IRT1135]
- National Natural Science Foundation of China [51006046, 51203064, 21201083, 51163014]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Industry-Academia-Research Joint Innovation Fund of Jiangsu Province [BY2012068]
- Science and Technology Support Program of Jiangsu Province [SBE201201094]
- Innovation Program for Graduate Education in Jiangsu Province [CXZZ13_07]
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Carbon nanofibers (CNFs) were prepared by combining electrospinning with a high-temperature carbonization technique. And a polyphenol biosensor was fabricated by blending the obtained CNFs with laccase and Nafion. Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscope (FE-SEM) were, respectively, employed to investigate the structures and morphologies of the CNFs and of the mixtures. Cyclic voltammetry and chronoamperometry were employed to study the electrocatalysis of the catechol biosensor. The results indicated that the sensitivity of the biosensor was 41 mu A.mM(-1), the detection limit was 0.63 mu M, the linear range was 1-1310 mu M and the response time was within 2 seconds, which excelled most other laccase-based biosensor reported. Furthermore, the biosensor showed good repeatability, reproducibility, stability and tolerance to interferences. This novel biosensor also demonstrated its promising application in detecting catechol in real water samples.
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