Journal
ACS COMBINATORIAL SCIENCE
Volume 13, Issue 3, Pages 218-222Publisher
AMER CHEMICAL SOC
DOI: 10.1021/co100024d
Keywords
combinatorial chemistry; mass spectrometry; proteomics; surface analysis; MALDI-MSI; inkjet printing
Funding
- Dutch Polymer Institute (DPI)
- Thuringer Kultusministerium [B515-07008]
- German Federal Ministry of Education and Research (BMBF)
- Interdisciplinary Center of Clinical Research (IZKF), Jena
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Taking advantage of the drop-on-demand capabilities of inkjet printing, the first example of a single tissue being used as a substrate for preparing combinatorial arrays of different matrix assisted laser desorption/ionization (MALDI) matrices in multiple concentrations on a single chip is reported. By varying the number of droplets per spot that were printed, a gradient array of different amounts of matrix material could be printed on a single chip, while the selection of matrices could be adjusted by switching different matrix materials. The result was a two-dimensional army of multiple matrices on a single tissue slice, which could be analyzed microscopically, and by MALDI to elucidate which combination of matrix and printing Conditions offered the best resolution in terms of spot-to-spot distance and signal-to-noise ratios for proteins in the recorded MS spectra. This combinatorial approach enables the efficient optimization of possible matrices in an organized, side-by-side array format, which can particularly be useful for the detection of specific protein markers.
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