Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41467-018-05190-9
Keywords
-
Categories
Funding
- National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre, from Addenbrooke's Hospital
- MRC core grant [RG84369]
- NIHR Research Professorship [RG67258]
- Cancer Research UK [RG66287]
- CRUK
- CRUK [C44943/A22536]
- SU2C [SU2C-AACR-DT1213]
- Wellcome Trust [102696]
- MRC [MC_UU_12022/2] Funding Source: UKRI
Ask authors/readers for more resources
Esophageal adenocarcinoma (EAC) incidence is increasing while 5-year survival rates remain less than 15%. A lack of experimental models has hampered progress. We have generated clinically annotated EAC organoid cultures that recapitulate the morphology, genomic, and transcriptomic landscape of the primary tumor including point mutations, copy number alterations, and mutational signatures. Karyotyping of organoid cultures has confirmed polyclonality reflecting the clonal architecture of the primary tumor. Furthermore, subclones underwent clonal selection associated with driver gene status. Medium throughput drug sensitivity testing demonstrates the potential of targeting receptor tyrosine kinases and downstream mediators. EAC organoid cultures provide a pre-clinical tool for studies of clonal evolution and precision therapeutics.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available