Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-05235-z
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Funding
- OrgBIO Organic Bioelectronics [PITN-GA-2013-607896]
- Sense-of-Care OFET biosensors for point-ofcare applications [PITN-2012-GA-316845]
- PON SISTEMA (MIUR)
- Future in Research FLOW Dispositivi EGOFET flessibili a bassa tensione per la sicurezza in campo alimentare (Codice Pratica) projects [ML5BJ85]
- CSGI
- RECAS
- CINECA under the ISCRA initiative [HP10CJSIFE-AQP4-NT, HP10BGY23X-OAP-AQP4]
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Label-free single-molecule detection has been achieved so far by funnelling a large number of ligands into a sequence of single-binding events with few recognition elements host on nanometric transducers. Such approaches are inherently unable to sense a cue in a bulk milieu. Conceptualizing cells' ability to sense at the physical limit by means of highly-packed recognition elements, a millimetric sized field-effect-transistor is used to detect a single molecule. To this end, the gate is bio-functionalized with a self-assembled-monolayer of 1012 capturing anti-Immunoglobulin-G and is endowed with a hydrogen-bonding network enabling cooperative interactions. The selective and label-free single molecule IgG detection is strikingly demonstrated in diluted saliva while 15 IgGs are assayed in whole serum. The suggested sensing mechanism, triggered by the affinity binding event, involves a work-function change that is assumed to propagate in the gating-field through the electrostatic hydrogen-bonding network. The proposed immunoassay platform is general and can revolutionize the current approach to protein detection.
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