Journal
NATURE COMMUNICATIONS
Volume 5, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms4309
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Funding
- National Institutes of Health Common Fund in Structural Biology [P50 GM073197, P50 GM073210, R01 GM095583]
- NIGMS PSI: Biology [U54 GM094618, U54 GM094599, U54 GM094625]
- National Science Foundation [MCB 1021557, MCB 1120997]
- BioXFEL Science and Technology Center [NSF 1231306]
- Science Foundation Ireland [12/IA/1255]
- Helmholtz Gesellschaft
- Max Planck Gesellschaft
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1021557] Funding Source: National Science Foundation
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Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mu m-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
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