4.8 Article

S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway

Journal

NATURE COMMUNICATIONS
Volume 5, Issue -, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/ncomms6401

Keywords

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Funding

  1. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) PhD studtenship
  2. Biotechnology and Biological Sciences Research Council PhD studentship
  3. Biotechnology and Biological Sciences Research Council [BBH0009841]
  4. Royal Society Research Fellowship and Research [Uf090321, Rg110495]
  5. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Research [473090/2011-2]
  6. Biotechnology and Biological Sciences Research Council [BB/H000984/1, BB/D011809/1] Funding Source: researchfish
  7. BBSRC [BB/D011809/1, BB/H000984/1] Funding Source: UKRI

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Nitrogen assimilation plays a vital role in plant metabolism. Assimilation of nitrate, the primary source of nitrogen in soil, is linked to the generation of the redox signal nitric oxide (NO). An important mechanism by which NO regulates plant development and stress responses is through S-nitrosylation, that is, covalent attachment of NO to cysteine residues to form S-nitrosothiols (SNO). Despite the importance of nitrogen assimilation and NO signalling, it remains largely unknown how these pathways are interconnected. Here we show that SNO signalling suppresses both nitrate uptake and reduction by transporters and reductases, respectively, to fine tune nitrate homeostasis. Moreover, NO derived from nitrate assimilation suppresses the redox enzyme S-nitrosoglutathione Reductase 1 (GSNOR1) by S-nitrosylation, preventing scavenging of S-nitrosoglutathione, a major cellular bio-reservoir of NO. Hence, our data demonstrates that (S) NO controls its own generation and scavenging by modulating nitrate assimilation and GSNOR1 activity.

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