Journal
NATURE COMMUNICATIONS
Volume 5, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms5894
Keywords
-
Categories
Funding
- NIH/NINDS New Innovator [NIH IDP20D017782-01]
- President and Provost of California Institute of Technology
- Biology and Biological Engineering Division of California Institute of Technology
- Beckman Institute of Caltech
- Gordon and Betty Moore Foundation [GBMF2809]
- NIH [1R21MH103824-01, 1R01NS085910-01, 1R01AG047664-01]
- Institute for Collaborative Biotechnologies through grant from the U.S. Army Research Office [W911NF-09-0001]
- Human Frontiers in Science Program
- Mallinckrodt Foundation
- Pew Charitable Trust
- Michael J. Fox Foundation
- Kimmel Foundation
- Caltech-GIST
- HHMI
- Caltech Biology Division Training grant [NIH/NRSA 5T32GM07616]
- German Research Foundation (DFG) [EN 957/1-1]
Ask authors/readers for more resources
Probing the neural circuit dynamics underlying behaviour would benefit greatly from improved genetically encoded voltage indicators. The proton pump Archaerhodopsin-3 (Arch), an optogenetic tool commonly used for neuronal inhibition, has been shown to emit voltage-sensitive fluorescence. Here we report two Arch variants with enhanced radiance (Archers) that in response to 655 nm light have 3-5 times increased fluorescence and 55-99 times reduced photocurrents compared with Arch WT. The most fluorescent variant, Archer1, has 25-40% fluorescence change in response to action potentials while using 9 times lower light intensity compared with other Arch-based voltage sensors. Archer1 is capable of wavelength-specific functionality as a voltage sensor under red light and as an inhibitory actuator under green light. As a proof-of-concept for the application of Arch-based sensors in vivo, we show fluorescence voltage sensing in behaving Caenorhabditis elegans. Archer1's characteristics contribute to the goal of all-optical detection and modulation of activity in neuronal networks in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available