Journal
NATURE COMMUNICATIONS
Volume 4, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms3672
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Funding
- Human Frontiers Science Program Career Development Award
- ISF Bikura Institutional Research Grant Program
- ERC starting Grant [309788]
- NHGRI [1P50HG006193-01, U54HG003067]
- NIH Pioneer Award [DP1OD003958-01]
- Klarman Cell Observatory
- NCI [R01 CA93947, U01 CA1411508]
- NIH [P50 CA093459]
- NIH Ruth L. Kirschstein National Research Service awards for individual Postdoctoral Fellowships
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The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.
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