Journal
ONCOLOGY LETTERS
Volume 7, Issue 5, Pages 1711-1717Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2014.1911
Keywords
forkhead box O3; forkhead box protein M1; casticin; breast cancer; therapeutic targets
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Funding
- Scientific Research of Hunan Province the Administration Bureau of Traditional Chinese Medicine [2010081]
- Hunan Province Science and Technology Project [2011FJ4144]
- program for Excellent Talents in Hunan Normal University [ET13107]
- Construct Program of the Key Discipline of Basic Medicine in Hunan Province
- Research Fund for the Doctoral Program of Hunan Normal University [110656]
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Casticin is an active ingredient derived from Fructus Viticis, a traditional Chinese medicine. This study aimed to investigate the role of forkhead box 03 (FOXO3a) in breast cancer cells and examine the regulatory mechanisms of FOXO3a in response to casticin treatment of the cells by ELISA, flow cytometry, small interfering RNA (siRNA) transfection and western blot analysis. Casticin treatment induced apoptosis and reduced the expression of the transcription factor forkhead box protein M1 (FOXM1). In addition, FOXM1 repression induced by casticin treatment was associated with the activation of FOXO3a via increased dephosphorylation. Notably, silencing FOXO3a expression by siRNA-mediated gene knockdown attenuated casticin-mediated apoptosis. Collectively, these findings suggest that FOXO3a is a critical mediator of the inhibitory effects of casticin on apoptosis in breast cancer cells.
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